Solapur University Question Paper M.Sc. – I (Semester – II) Examination, 2014 MICROBIOLOGY (Paper – VI) (New CGPA Pattern) Microbial Physiology and Metabolism Day and Date : Thursday, 24-4-2014 Max. Marks : 70 Time : 11.00 a.m. to 2.00 p.m., N.B. : 1) Section – I is compulsory. 2) From Section – II attempt any four. 3) All questions carry equal marks. 4) Figures to the right indicate full marks. 5) Draw neat and labelled diagrams. SECTION – I 1. Rewrite the following sentences by using correct alternatives : 14 1) In anaerobic respiration terminal electron acceptor is ___________ a) O2 b) NO3 c) Pyruvic acid d) Succinic acid 2) ___________ carry electrons in respiratory electron transport system. a) NAD b) Co-enzyme c) Flavoprotein d) Cytochrome 3) Peroxidase catalyzes conversion of H2O2 to ___________ a) H2 and O2 b) H2 and 2 1 O2 c) H2O and 2 1 O2 d) H2 and − O2 4) ___________ is precursor for purine and pyrimidine synthesis. a) Phosphoribosyl amino imidazole. b) Phosphoribosyl glycinamide c) Phosphoribosyl carboxyamide d) Phosphoribosyl pyrophosphoric acid SLR-VO – 6 -2- 5) ___________ phosphate molecules are taken up for NAD linked electron transport chain. a) 1 b) 2 c) 3 d) 4 6) ___________ are the channel proteins involved in transport across cell membrane. a) Prostaglandins b) Porins c) Porphyrins d) Interleukins 7) ___________ shows similarities with probable ancestor of mitochondria. a) Diplococcus b) Micrococcus c) Paracoccus d) Mixococcus 8) A revolving door model of active transport has been proposed to explain passage of ___________ through cell membrane. a) Glucose b) Maltose c) Galactose d) Lactose 9) Phycobilins are water soluble open chain ___________ a) Tetraphytols b) Phytols c) Tetra pyrroles d) Octapyrroles 10) ___________ can takes place across the concentration gradient and requires energy. a) Simple diffusion b) Passive diffusion c) Facilitated diffusion d) None of these 11) Cytochromes are ___________ a) Lipids b) Carbohydrates c) Fats d) Proteins 12) TCA cycle is a major route of ATP generation in ___________ a) Chemolithotrophs b) Chemoheterotrophs c) Phototrophs d) Chemoautotrophs -3- SLR-VO – 6 13) Cytochrome pattern of ___________ changes as growth phases changes. a) Arthrobacter b) Acetobacter c) Azotobacter d) Aeromonas 14) ___________ enzyme is involved in aspartate synthesis from oxaloacetate. a) Glutamate aspartate transaminase b) Glutamine aspartate transaminase c) Oxaloacetate aspartate transaminase d) Aspartic acid transaminase SECTION – II Attempt any four : 2. Write an essay on ‘transport mechanism’. 14 3. Write in detail Denovo synthesis of purines. 14 4. Write an essay on ‘TCA cycle’. 14 5. Write in detail about ‘Bacterial electron transport chain’. 14 6. Answer any two of the following : 14 i) Mechanism of oxygen toxicity ii) Reverse osmosis iii) Microbial response to stress. 7. Answer any two of the following : 14 i) Explain in detail about peroxidase and superoxide dismutase. ii) Write on synthesis of fatty acids. iii) Explain in detail role of pyruvate dehydrogenase in TCA cycle. _____________________
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